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Horseradish Peroxidase Nanopatterned Electrodes by Click Chemistry: Application to the Electrochemical Detection of Paracetamol |
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| Authors: | Andreea Cernat Sophie Griveau Cyrille Richard Fethi Bedioui Robert Săndulescu |
| Institution: | 1. Chimie ParisTech, Ecole Nationale Supérieure de Chimie de Paris, Unité de Pharmacologie Chimique et Génétique et Imagerie, Paris, France;2. CNRS, Unité de Pharmacologie Chimique et Génétique et Imagerie UMR 8151, Paris, France;3. Université Paris Descartes, Sorbonne Paris Cité, Unité de Pharmacologie Chimique et Génétique et Imagerie, Paris, France;4. INSERM, Unité de Pharmacologie Chimique et Génétique et Imagerie (N° 1022), Paris, France;5. University of Medicine and Pharmacy, Faculty of Pharmacy, Analytical Chemistry Dept., 4 Pasteur St. 400349, Cluj‐ Napoca, Romania |
| Abstract: | We propose the fabrication of nanostructured glassy carbon (GC) electrodes modified with horseradish peroxidase (HRP) for the detection of paracetamol. This was accomplished by inducing the nanostructuration of GC via the adsorption of polystyrene nanospheres (900 nm diameter) followed by electropolymerization of N‐(10‐azidodecyl)pyrrole. The nanospheres were then removed and nanostructured polypyrrole‐GC was submitted to click reaction in presence of ethynyl‐biotin that was further coupled to HRP‐avidin. The electrode was then used to sense the electrochemical reduction of the enzymatically generated electroactive oxidized species of acetaminophen (NAPQI) in the presence of hydrogen peroxide. The nanostructured electrode with HRP exhibits a fast response towards NAPQI reduction and improved performances in terms of sensitivity and limit of detection compared to non structured electrode. |
| Keywords: | Nanolithography Nanostructuration HRP Click chemistry Enzymatic reaction |