Electrochemical Behaviors of Glucose Oxidase Based on Biocatalytic Deposition of Gold Nanoparticles |
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Authors: | Yaping He Jianbin Zheng |
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Institution: | Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, China |
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Abstract: | Coupling nanotechnology with biocatalysis, a highly sensitive glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using monkshoodvine root–bark like carbon (MLC) as the platform for the biocatalytic deposition of AuNPs. The biosensor showed a linear range from 0.25 to 130 μM with a detection limit of 0.1 μM (S/N = 3) towards glucose and sensitivity of 3010 μA/mM. K value was calculated to be 67.4 μM. Furthermore, the proposed AuNPs/GOx–MLC modified pyrolytic graphite electrode (AuNPs/GOx–MLC/PGE) achieved direct electron transfer of GOx. Γ* was calculated to be 2.79 × 10?11 mol/cm2 and ks was 1.79 s?1. It also showed a remarkable electrocatalysis towards glucose. |
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Keywords: | Biocatalytic deposition Electrochemical behaviors Glucose oxidase Monkshoodvine root– bark like carbon Au nanoparticles |
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