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Fluorometric investigation of the interaction of bovine serum albumin with surfactants and 6-mercaptopurine |
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| Authors: | Hu Yan-Jun Liu Yi Jiang Wei Zhao Ru-Ming Qu Song-Sheng |
| Affiliation: | aDepartment of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China bState Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China cDepartment of Chemistry, Tsinghua University, Beijing 100084, PR China |
| Abstract: | Fluorescence quenching in solutions of bovine serum albumin has been investigated in the presence of 6-mercaptopurine and ionic surfactants. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of bovine serum albumin by 6-mercaptopurine was dynamic quenching mechanism. The Stern–Volmer quenching model has been successfully applied, and the activation energy of the interaction between 6-mercaptopurine and bovine serum albumin as much as 4.26 kJ mol−1 was calculated. The distance r between donor (bovine serum albumin) and acceptor (6-mercaptopurine) was obtained according to fluorescence resonance energy transfer (FRET). The result of synchronous fluorescence spectra shows that the conformation of bovine serum albumin has been changed at the present of 6-mercaptopurine. |
| Keywords: | 6-Mercaptopurine Bovine serum albumin Fluorescence spectroscopy UV–Visible spectroscopy Ionic surfactant Fluorescence resonance energy transfer |
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