Fluctuation in the ergosterol and deoxynivalenol content in barley and malt during malting process |
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Authors: | Vlastimil Dohnal Alena Jezkova Lucie Pavlikova Kamil Musilek Daniel Jun Kamil Kuca |
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Institution: | 1. Department of Chemistry, Faculty of Science, University of J.E. Purkynje in Usti nad Labem, Ceske mladeze 8, 400 96, Usti nad Labem, Czech Republic 2. Department of Toxicology, Faculty of Military Health, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic 3. Department of Food Technology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemedelská 1, 613 00, Brno, Czech Republic 4. Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic
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Abstract: | This paper describes determination of the deoxynivalenol and ergosterol in samples from different varieties of barley and,
consequently, malt produced from this barley. In total, 20 samples of barley and 20 samples of barley malt were analyzed.
The alkaline hydrolysis with consequent extraction into hexane was applied to obtain the ergosterol from cereals. Extraction
to acetonitrile/water and subsequent solid-phase extraction (SPE) were used for deoxynivalenol. The determination of the samples
was performed on high-performance liquid chromatography using UV detection (ergosterol) and mass spectrometric detection (deoxynivalenol).
The influence of the malting process on the production of two compounds of interest was assessed from obtained results. Ergosterol
concentration ranged 0.88–15.87 mg/kg in barley and 2.63–34.96 mg/kg in malt, where its content increased to 95% compared
to samples before malting. The malting process was observed as having a significant effect on ergosterol concentration (P = 0.07). The maximum concentration of deoxynivalenol was found to be 641 μg/kg in barley and 499 μg/kg in malt. Its concentration
was lower than the legislative limit for unprocessed cereals (1,250 μg/kg). The statistic effect of the malting process on
deoxynivalenol production was not found. Linear correlation between ergosterol and deoxynivalenol content was found to be
very low (barley R = 0.02, malt R = 0.01). The results revealed that it is not possible to consider the ergosterol content as the indicator of deoxynivalenol
contamination of naturally molded samples. |
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