BIOLUMINESCENCE OF THE BRITTLE STAR OPHIOPSILA CALIFORNICA |
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Authors: | Osamu Shimomura |
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Institution: | Marine Biological Laboratory, Woods Hole, MA 02543, USA |
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Abstract: | The light-emitting principle of the brittle star Ophiopsila californica has been isolated and purified. It was found to be a green-fluorescent photoprotein (molecular weight 45000) which emits green light (λmax 500 nm) when H2O2 is added, independently of the presence or absence of O2. The green fluorescence (emission maximum 500 nm, excitation maximum 440 nm) spectrally coincided with the H2O2-triggered luminescence, indicating that the green fluorescent chromophore is the light-emitter of the photoprotein luminescence. |
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