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Studying dispersoid systems
Authors:G V Kotelnikov  Sophia P Moiseyeva  E V Mezhburd  E I Maevsky  Elena V Grishina
Institution:(1) Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Russia;(2) Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Russia;(3) Institute for Biological Instrumentation, Russian Academy of Sciences, 142290 Pushchino, Russia;(4) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia;(5) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia
Abstract:Summary Measurements of energy transformation in mitochondria are done on a capillary differential titration calorimeter CTD2156. It is important to mention that a sediment is quickly formed by the mitochondria suspension without mixing by means of a vibrating needle. During the measurements, the vibrating needle is located inside the working volume of the chamber. The design of the calorimeter is substantiated theoretically. It provides a new mode of a reagent input in the measuring volume of the calorimetric chambers. It expands the spectrum of tasks that can be solved using this instrument. In the capillary calorimeter the calorimetric chambers unit is simple and small in size. These advantages of capillary chambers provide an opportunity to unite 20 capillary calorimetric chambers in one calorimetric block. It allows designing a multi-channel titration calorimeter. There are obvious advantages of such a calorimeter over other instruments in screening researches and in researches of objects maintaining stability only for a short time.
Keywords:differential titration calorimeter  mixing reagents in a capillary chamber  sensitive volume of calorimetric chambers  mitochondria  capillary calorimetric chamber  heat-conducting bridge
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