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Evaluation of antioxidant activities of the edible and medicinal Acacia albida organs related to phenolic compounds
Authors:Samira Karoune  Hanen Falleh  Mohamed Seif Allah Kechebar  Youcef Halis  Khaoula Mkadmini  Mohamed Belhamra
Institution:1. Laboratoire d'Ecotoxicologie et stress abiotiques, Dépt Biologie et Ecologie, Faculté SNV, Université Constantine 1, Constantine, Algeria;2. Centre de Recherche Scientifique et Technique sur les Régions Arides, Campus Universitaire, BP 1682 R.P., Biskra07000, Algeria;3. Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj-Cédria, BP 901, 2050Hammam-lif, Tunisia;4. Centre de Recherche Scientifique et Technique sur les Régions Arides, Campus Universitaire, BP 1682 R.P., Biskra07000, Algeria;5. Laboratoire Diversité des Ecosystèmes et Dynamiques des Systèmes de Production Agricoles en Zones Arides, Dépt d'agronomie, Université Mohamed Khider, Biskra07000, Algeria
Abstract:This study compared phenolic contents and antioxidant activity in different organs of Acacia albida (leaves and bark) and focuses on identification of phenolic compounds of leaves by HPLC-DAD. The analysed organs exhibited differences in total polyphenol contents (100 and 59.5 mg GAE g? 1 DW). Phenolic contents of leaves were two times higher than those in bark. Ethanolic extracts exhibited good antioxidant activities with IC50 = 26 μg mL? 1 for DPPH and EC50 = 50 μg mL? 1 for FRAP. Identification by HPLC-DAD revealed the presence of nine phenolic compounds known for their high antioxidant activity. The results suggested that this species can be used as source of natural antioxidants.
Keywords:Acacia albida  phenolic compounds  HPLC-DAD  leaves  bark
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