Comparative evaluation of two Trichoderma harzianum strains for major secondary metabolite production and antifungal activity |
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Authors: | Vivek Ahluwalia Jitendra Kumar Virendra S Rana Om P Sati S Walia |
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Institution: | 1. Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi110 012, India;2. Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal), Uttarakhand246 174, India;3. Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal), Uttarakhand246 174, India |
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Abstract: | This investigation was undertaken to identify the major secondary metabolite, produced by two Trichoderma harzianum strains (T-4 and T-5) with their antifungal activity against phytopathogenic fungi using poison food technique. The ethyl acetate extract was subjected to column chromatography using n-hexane, ethyl acetate and methanol gradually. Chromatographic separation of ethyl acetate extract of T. harzianum (T-4) resulted in the isolation and identification of palmitic acid (1), 1,8-dihydroxy-3-methylanthraquinone (2), 6-pentyl-2H-pyran-2-one (3), 2(5H)-furanone (4), stigmasterol (5) and β-sitosterol (6), while T. harzianum (T-5) gave palmitic acid (1), 1-hydroxy-3-methylanthraquinone (7), δ-decanolactone (8), 6-pentyl-2H-pyran-2-one (3), ergosterol (9), harzianopyridone (10) and 6-methyl-1,3,8-trihydroxyanthraquinone (11) as major metabolites. Among compounds screened for antifungal activity, compound 10 was found to be most active (EC50 35.9–50.2 μg mL? 1). In conclusion, the present investigation provided significant information about antifungal activity and compounds isolated from two different strains of T. harzianum obtained from two different Himalayan locations. |
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Keywords: | Trichoderma secondary metabolite antifungal activity |
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