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Electrochemical detection of a Vibrio parahaemolyticus sequence-specific gene based on a gold electrode modified with a single stranded probe |
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| Authors: | MinBo Lan Qin Zhou YanHui Zhao YuanJie Teng Chen Chen HongLi Zhao HuiHui Yuan |
| Institution: | 1. Key Laboratory for Advanced Materials and Analysis Test Research Center, East China University of Science and Technology, Shanghai, 200237, China 2. Key Laboratory for Ultrafine Materials, Ministry of Education, East China University of Science and Technology, Shanghai, 200237, China |
| Abstract: | An electrochemical DNA biosensor for specific-sequences detection of Vibrio parahaemolyticus (VP) was fabricated. A single-stranded 20-mer oligonucleotide (ssDNA) and 6-mercapto-1-hexanol (MCH) were immobilized via a thiol linker on gold disk electrodes by self-assembling. The ssDNA underwent hybridization in a hybridization solution containing complementary or non-complementary or single base pair mismatched DNA sequences of VP. Examination of changes in response to these three target DNAs showed that the developed biosensor had a high selectivity and sensitivity. |
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