| 水溶性凝胶渗透色谱测定芸芝多糖各组分的分子量及相对含量 |
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| 引用本文： | 李静,左雄军.水溶性凝胶渗透色谱测定芸芝多糖各组分的分子量及相对含量[J].广州化学,1998(4). |
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| 作者姓名： | 李静 左雄军 |
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| 作者单位： | 中国科学院广州化学研究所!广州，510650，中国科学院广州化学研究所!广州，510650 |
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| 基金项目： | 中国科学院广州分院测试基金 |
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| 摘 要： | 用水溶性凝胶渗透色谱法研究了芸芝多糖在水溶性凝胶柱上的吸附影响因素，建立了最佳分离、测试条件，成功地将芸芝多糖的混合物分离为三个组分，并测试各组分的分子量分别为1190000；796000；552000。各组分相对百分含量分别为83．6％，8．9％，7．5％。
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| 关 键 词： | 水溶性凝胶渗透色谱法 芸芝多糖 吸附 分子量 相对含量 |
Determination of molecular weight and relative content of the Component of polysaccharide krestin with aqueous gel permeation chromatography |
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| Li jing, Zhuo xongjun.Determination of molecular weight and relative content of the Component of polysaccharide krestin with aqueous gel permeation chromatography[J].Guangzhou Chemistry,1998(4). |
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| Authors: | Li jing Zhuo xongjun |
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| Abstract: | The Polysaccharide Krestin is a mixture of Proteoglycan , it is obtained from coriolus versiolor in Basidimycete , It has the medical performance of anti - caneer, conteract inflammation , enhancement of the system and inhibition, With aqueous gel permeation chromatography , we discuss the effect of mobile phase to the adsorption of the PSK on the Ultrahydrogelcoumn. We find that the optimum ion strength of the mobile phase is 0.05mol/L NaNO3 . Theoptimum condition of separation and determination are established. The results show that the PSKis made of three fractins . Molecular weight and relative content of the first 、the second、the thirdcomponent of the PSK are 1190000, 83. 6%;796000, 8. 9%;552000,7.5% respectively. |
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| Keywords: | Aqueous Gel Permeation Chromatography polysaccharide Krestin adsorption molecular weight relative content |
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