Development of a chromatographic method for the determination of saquinavir in plasma samples of HIV patients |
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Authors: | Campanero M A Escolar M Arangoa M A Sádaba B Azanza J R |
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Affiliation: | Department of Clinical Pharmacology, Clinica Universitaria de Navarra, Pamplona, Spain. macampaner@unav.es |
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Abstract: | A simple, sensitive and reproducible high-performance liquid chromatographic method for detecting and quantifying saquinavir in human plasma is described. Verapamil was used as internal standard. The method employes a single liquid-liquid extraction step with tert-butil methyl ether followed by chromatography on a Lichrospher 60 Select B C8 reversed-phase column. Ultraviolet detection was used to identify the compounds of interest. The quantitation limit of saquinavir was 1 ng/mL and only 0.5 mL of plasma sample was required for the determination. The average saquinavir recoveries over a concentration range of 2.5-500 ng/mL ranged from 86 to 95%. Precision and accuracy did not exceed 5%. |
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