Disturbance-free digital holographic microscopy via a micro-phase-step approach |
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| Institution: | 1. Department of Electro-optical and Energy Engineering, Ming Dao University, Changhua 52345, Taiwan, ROC;2. School of Defense Science, National Defense University, Tashi, Taoyuan 33509, Taiwan, ROC;3. Department of Applied Chemistry & Material Science, National Defense University, Taiwan, ROC;4. Department of Material Science and Engineering, Ming Dao University, Changhua 52345, Taiwan, ROC;1. Biomedical Engineering Department, Konyang University, Nonsan, Chung-Nam 320-711, Republic of Korea;2. Realistic Broadcasting Media Department, Electronics and Telecommunications Research Institute, Daejeon 305-700, Republic of Korea;1. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, PR China;2. Yaroslav-The-Wise Novgorod State University, Veliky Novgorod 173003, Russian Federation;3. Institute of Optical Monitoring of Climatic and Ecological System SB RAS, Tomsk 634055, Russian Federation;1. MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China;2. Key Laboratory of Micro-systems and Micro-structures Manufacturing of Ministry of Education, Harbin Institute of Technology, Harbin 150001, China;3. Yaroslav-the-Wise Novgorod State University, Veliky Novgorod 173003, Russian Federation;4. Institute of Monitoring of Climatic and Ecological Systems SB RAS, Tomsk 634055, Russian Federation;1. Department of Automation, North China Electric Power University, Baoding 071003, China;2. School of Electrical and Electronic Engineering, The University of Manchester, Manchester M13 9PL, UK |
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| Abstract: | This work proposes a cost-effective, simple, micro-phase-step (MPS) method for suppressing the zero-order diffraction and conjugate-image interferences that are caused during digital holographic microscopic image reconstruction. The proposed MPS method replaces the conventional phase modulation approach; it uses a rotatable cover glass that enables smooth modification of the incidence angle and the optical path of the reference beam. This setup allows the phase step to be accurately estimated by shifting the reference wave phase more freely close to π/2, at which the background noise can be suppressed more effectively. In the proposed MPS method, the optimal conditions for suppressing conjugate-image interference are identified using a relatively moderate intensity distribution and suppression of noise in the numerically reconstructed object wave-field. In addition, the proposed method mitigates the effect of disturbances that are caused by environmental factors, such as minor vibrations and small changes in temperature and humidity. Importantly, only two holograms are required to satisfy the objective of image reconstruction. The results in this work reveal that even with intentional interference caused by minor vibrations, conjugate-image interference can still be suppressed by determining the phase deviation between the two original holograms. |
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| Keywords: | Digital holographic microscopy Micro-phase-step Hologram |
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