| 蛋白质测定的流动注射荧光法 |
| |
| 引用本文： | 宋功武,方光荣,刘立明,李玲,蔡朝霞. 蛋白质测定的流动注射荧光法[J]. 分析测试学报, 2002, 21(3): 56-58 |
| |
| 作者姓名： | 宋功武 方光荣 刘立明 李玲 蔡朝霞 |
| |
| 作者单位： | 湖北大学分析测试中心,湖北武汉,430062;湖北大学分析测试中心,湖北武汉,430062;湖北大学分析测试中心,湖北武汉,430062;湖北大学分析测试中心,湖北武汉,430062;湖北大学分析测试中心,湖北武汉,430062 |
| |
| 基金项目： | 中国分析测试基金资助课题(99-11) |
| |
| 摘 要： | 根据荧光素的单体－二聚体平衡转化的原理,提出了一种检测蛋白质的方法;荧光素在表面活性剂作用下形成二聚体后荧光强度明显降低,加入蛋白质后二聚体解聚使荧光强度增加;结合流动注射法测定牛血清蛋白（BSA）,该法简便,灵敏度高,对血清中蛋白质进行测定,结果令人满意。
|
| 关 键 词： | 二聚体 荧光素 牛血清蛋白 流动注射分析 荧光法 |
| 文章编号： | 1004-4957(2002)03-0056-03 |
| 修稿时间： | 2001-06-12 |
Determination of Protein by Flow Injection Fluorometry with Fluorescein |
| |
| SONG Gong _ wu,FANG Guang _ rong,LIU Li _ ming,LI Ling,CAI Zhao _ xia. Determination of Protein by Flow Injection Fluorometry with Fluorescein[J]. Journal of Instrumental Analysis, 2002, 21(3): 56-58 |
| |
| Authors: | SONG Gong _ wu FANG Guang _ rong LIU Li _ ming LI Ling CAI Zhao _ xia |
| |
| Abstract: | A flow injection fluorometry for the determination of protein is reported based on shifting the dimer- monomer equilibrium of fluorescein. Fluorescence intensity of fluorescein solution decreases when the surfactant of cetyltrimethylammonium bromide is added with formation of dimer of fluorescein, and the fluorescence of the solution enhances again when protein is added with depolymerization of the dimer. The method has the advantages of simplicity and high sensitivity. It has been satisfactorily employed for the determination of BSA in serum samples. |
| |
| Keywords: | Dimer Fluorescein BSA Flow injection analysis Fluorescence method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
