交联酶聚体技术研究进展*

交联酶聚体（CLEAs）是一类新型的固定化酶技术，具有制备简单、酶活回收率高、操作和保存稳定性强等优点。近年来，CLEAs技术与材料学、印迹工程、介质工程、反应工程学等相结合取得了一系列新进展，包括载体固定化CLEAs、包埋CLEAs、印迹法CLEAs、多酶CLEAs、CLEAs膜浆反应器等，在手性分子拆分与合成。抗生素生产等领域取得了一些成果。本文对CLEAs酶活影响因素及CLEAs技术的最新研究进展进行了分析与总结，并展望了需进一步深入开展的内容，有助于生物工程、酶工程、化学工程和材料科学等领域相关研究工作的开展。

Advances in Cross-Linked Enzyme Aggregates

Cross-linked enzyme aggregates (CLEAs) is a novel kind of carrier-free immobilized enzyme. It has many advantages including simple preparation procedure, high catalytic activity, easy recovery and increased stability. In recent years, a series of new progress has been made in preparation of CLEAs through combining material science, molecular imprinting engineering, medium engineering and reaction engineering with enzyme immobilization. Car-rier-immobilized CLEAs, encapsulated CLEAs, imprinted CLEAs, combi-CLEAs and CLEAs membrane slurry reactor are respectively discussed, and their application in chiral separation and antibiotics production are introduced. Important factors influencing the activity of CLEAs are analyzed and most recent developments of CLEAs are summarized in this paper. In addition, some prospects for this attractive technology are also presented. It will be helpful for relevant researches in bioengineering, enzyme technology, chemical engineering and materials science. Contents
1 Introduction
2 Important factors influencing the activity of CLEAs
3 Progress in CLEAs
3.1 Carrier- immobilized CLEAs
3.2 Encapsulated CLEAs
3.3 Imprinted CLEAs
3.4 Combi-CLEAs
3.5 CLEAs membrane slurry reactor
4 Applications in biocatalysis and biotransformtion
5 Problems and prospects