Kinetic analysis of interaction between lipopolysaccharide and biomolecules

Lipopolysaccharide (LPS) is a major component of the outer membrane of all gram-negative bacteria. It interacts with some biomolecules and triggers a toxic reaction. In this paper, we studied the interaction between LPS from Salmonella Minnesota and some biomolecules using a surface plasmon resonance (SPR) biosensor. Biomolecules were immobilized on a CM5 sensor chip using the amino coupling method and LPS was injected over the immobilized surfaces. The affinity constant K_A of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 10⁷, 2.03 × 10⁸, 7.58 × 10⁶, 2.82 × 10⁴ L·mol⁻¹, respectively. However, LPS could not interact with ferritin. __________ Translated from Chinese Journal of Analytical Chemistry, 2007, 35(5): 677–680 [译自: 分析化学]