SPECTROSCOPIC AND ORIENTATIONAL PROPERTIES OF CHLOROPHYLL a AND CHLOROPHYLL b IN LIPID MEMBRANES |
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| Authors: | M VAN GURP† U VAN DER HEIDE J VERHAGEN T PITERS G VAN GINKEL Y K LEVINE |
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| Institution: | Department of Molecular Biophysics, Buys Ballot Laboratory, University of Utrecht, P.O. Box 80.000, 3508 TA Utrecht, The Netherlands |
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| Abstract: | Abstract
Linear dichroism and fluorescence depolarization measurements on chlorophyll a and chlorophyll b molecules incorporated into macroscopically oriented multibilayers of the plant lipid digalactosyldiacylglycerol (DGDG) are presented. The results are combined with measurements of fluorescence anisotropy to obtain both the order parameters describing the orientational statistics and the directions of the absorption and emission transition moments in the frame of the molecules. The problem presented by the overlapping nature of the absorption and emission bands is overcome by determining the fluorescence depolarization at the two maxima of the emission spectrum. |
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