微孔尼龙-6膜的催化水解改性和DNA芯片的原位合成

基因芯片常用的载体有玻璃片、硅片、聚丙烯膜、硝酸纤维膜和尼龙膜等1～ 4 ] .其中有机高分子膜载体由于自身荧光背景较强 ,寡核苷酸探针或 c DNA片段通常用点样法固定 ,并以同位素标记的靶基因与其杂交 ,用放射显影进行检测 ,探针密度不高且损害人体健康 .生物分子纳米标记和时间分辨荧光等检测技术可以非常有效地克服载体荧光背景5,6 ] .因此 ,若将高分子材料进行改性 ,使其表面带有羟基、氨基和巯基等活性基团并应用于 DNA的原位合成 ,再结合新开发的生物分子标记和检测方法 ,将拓展生物芯片基材选择范围 ,并开发出新的生物芯片制…

Catalytic Hydrolysis and Modification of Microporous Nylon-6 Membranes and Insite Synthesis of DNA Chip Active Membranes

Microporous polyamide-6 membranes were hydrolyzed in 0.01mol/L NaOH/CH₃OH medium by refluxing for about 36h.By investigating the UV spectra, the hydrolyzing conditions were optimized.ApH value of 12 and reaction time of 36h are the preferred conditions for hydrolysis.The treated membrane can be applied for in site synthesis of oligonucleotide and, for example, the oligonucleotide probes of 5′-AACCACCAAACACAC-3′ were successfully synthesized on the hydrolyzed membrane,which was proved by the high resolution X-ray photoelectron spectroscopy(XPS) of P_2pThe single step coupling efficiency was determined by UVspectra to be in excess of 98%.