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Glucose oxidase as a blocking agent-based signal amplification strategy for the fabrication of label-free amperometric immunosensors |
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| Authors: | SONG ZhongJu YUAN Ruo CHAI YaQin CHE Xin&LV Ping Key Laboratory on Luminescence Real-Time Analysis Ministry of Education College of Chemistry Chemical Engineering Key Laboratory of Eco-environments in Three Gorges Reservoir Region Southwest University Chongqing China |
| Institution: | SONG ZhongJu,YUAN Ruo *,CHAI YaQin,CHE Xin&LV Ping Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,College of Chemistry and Chemical Engineering,Key Laboratory of Eco-environments in Three Gorges Reservoir Region,Southwest University,Chongqing 400715,China |
| Abstract: | An effective electrochemical signal amplification strategy based on enzyme membrane modification and redox probe immobilization was proposed to construct an amperometric immunosensor.L-cysteine@ferrocene functionalized chitosan,which possessed not only efficient redox-activity but also excellent film-forming ability,was coated on the bare glass carbon electrode. Moreover,the thiol groups(SH)in the ferrocenyl compound were used for gold nanoparticles immobilization via the strong bonding interaction,which co... |
| Keywords: | amperometric immunosensor chitosan gold colloidal nanoparticles(GNPs) Ferrocene-monocarboxylic(Fc?COOH) carcinoembryonic antigen(CEA) |
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