Computer-assisted optimization for the selection of mobile phase in semi-preparative HPLC

A computer-assisted method is presented for optimization for the selection of mobile phasecomposition in semi-preparative HPLC.The optimization for the expected separation is based ona polynomial estimation from five preliminary runs.Statistical scanning technique was used foroptimization.Double criteria simulation system (DCSS) is established for chromatographic perfor-mance measurement in this method.The validity of the optimization strategy is confirmed by applyingit to a technical Cypermethrin separation.Excellent agreement is obtained between the predictedand experimental results.

Computer-assisted optimization for the selection of mobile phase in semi-preparative HPLC

A computer-assisted method is presented for optimization for the selection of mobile phase composition in semi-preparative HPLC. The optimization for the expected separation is based on a polynomial estimation from five preliminary runs. Statistical scanning technique was used for optimization. Double criteria simulation system (DCSS) is established for chromatographic performance measurement in this method. The validity of the optimization strategy is confirmed by applying it to a technical Cypermethrin separation. Excellent agreement is obtained between the predicted and experimental results.