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Pre-column derivatization of rofecoxib for determination in serum by HPLC |
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| Authors: | M?Amini M?PirAli?Hamedani M?Vosooghi M?Nabavi |
| Institution: | (1) Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran |
| Abstract: | An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid–liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10–500 ng ml–1. |
| Keywords: | Rofecoxib HPLC Serum UV detection |
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