Microfluidic chip-based aptasensor for amplified electrochemical detection of human thrombin |
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Authors: | Huixiang Wang Yun Liu Chengcheng Liu Jingyu Huang Pengyuan Yang Baohong Liu |
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Institution: | Department of Chemistry, Institute of Biomedical Sciences, Fudan University, Shanghai 200433, People’s Republic of China |
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Abstract: | In this paper, a microchip-based sandwich-type aptasensor is developed for the detection of human thrombin. The SH-aptamer/thrombin/alkaline phosphatase-functionalized aptamer (ALP-aptamer) system was constructed in the microfluidic channels. And the substrate solution containing 4-aminophenyl phosphate (p-APP) was introduced to the microchannels for the end-column electrochemical detection. The on-chip aptasensor has a broad linear response range of 1–100 pM with a detection limit of 1 pM, which shows high sensitivity and specificity. The system was then applied to detect thrombin in human serum sample. Therefore, the on-chip aptasensor has a great promise for detecting and screening ultratrace levels of biomarkers in the complex matrices. |
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