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A new strategy for the preparation of maleimide-functionalised gold surfaces |
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| Authors: | Xin Zhang Guoguang Sun Marc Hovestädt Vitali Syritski Norbert Esser Rudolf Volkmer Silvia Janietz Jörg Rappich Karsten Hinrichs |
| Institution: | 1. Helmholtz-Zentrum Berlin für Materialien und Energie, Institut für Si-Photovoltaik, Kekuléstr. 5, 12489 Berlin, Germany;2. Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Department Berlin, Albert-Einstein-Str. 9, 12489 Berlin, Germany;3. Institut für medizinische Immunologie, Charité Berlin, Hessische Str. 3-4, Berlin, Germany;4. Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia;5. Fraunhofer-Institut für Angewandte Polymerforschung, Geiselbergstr. 69, 14476 Potsdam, Germany |
| Abstract: | We have developed and investigated a new route to functionalise Au surfaces using maleimide groups. This functionalisation has been performed by grafting aminophenyl (AP) via an electrochemical reduction of 4-aminophenyldiazonium salt in acetonitrile solution and the subsequent chemical binding of N-(2-carboxyethyl) maleimide (NCEM). The resulting maleimide functionalised surface was interacted with a cysteine-modified peptide. The grafting of AP was monitored by the occurrence of NH2 and aryl ring vibrations, whereas the binding of the NCEM led to a strong and sharp peak because of the C═O stretching mode. The immobilisation of the peptide was identified by the appearance of the amide I band. Half of the maleimide surface groups reacted with the peptide because of steric hindrance. The charge efficiency for the AP layer formation was about 45% at a thickness of about 6–8 nm. |
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