DNA interactions and anticancer screening of copper(II) complexes of <Emphasis Type="Italic">N</Emphasis>-(methylpyridin-2-yl)-amidino-<Emphasis Type="Italic">O</Emphasis>-methylurea |
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| Authors: | Romrawee Pratumwieng Atittaya Meenongwa Rosa F Brissos Patrick Gamez Yanee Trongpanich Unchulee Chaveerach |
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| Institution: | 1.Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Materials Chemistry Research Center,Khon Kaen University,Khon Kaen,Thailand;2.Chemistry Division, Department of Science, Faculty of Science and Technology,Rajamangala University of Technology Krungthep,Bangkok,Thailand;3.Departament de Química Inorganica,Universitat de Barcelona,Barcelona,Spain;4.Institució Catalana de Recerca I Estudis Avan?ats (ICREA),Barcelona,Spain;5.Department of Biochemistry, Faculty of Science,Khon Kaen University,Khon Kaen,Thailand |
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| Abstract: | Cu(II) complexes of the tridentate ligand N-(methylpyridin-2-yl)-amidino-O-methylurea (L), namely Cu(L)Cl2] and Cu(L)ClO4]ClO4, have been investigated for interactions with DNA by spectroscopic methods and viscosity measurements. Both complexes bind to DNA through non-intercalative interactions. Cu(L)Cl2] (K b = 2.81 × 105 M?1) shows similar DNA-binding potential to Cu(L)ClO4]ClO4 (K b = 1.57 × 105 M?1). Investigation of the chemical nuclease properties toward plasmid pBR322 DNA by gel electrophoresis and atomic force microscopy (AFM) suggests that both complexes are able to cleave the supercoiled form (Form I) to the nicked (Form II) and linear forms (Form III) through an oxidative pathway. The possible reactive oxygen species have been investigated by the use of scavengers, indicating that hydroxyl radicals may be involved in the DNA cleavage mechanism. Both of these complexes show similar activities against selected human cancer cell lines. |
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