Multi-Residue Analysis of Avermectins in Bovine Liver and Muscle by Liquid Chromatography–Fluorescence Detector |
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| Authors: | Xiao lin Hou Yongnin Wu Jianzhong Shen Lei Wang Shuangyang Ding |
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| Institution: | (1) Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, People’s Republic of China;(2) Institute of Nutrition and Food Hygiene, Chinese Center for Disease Control and Prevention, Beijing, 100050, People’s Republic of China |
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| Abstract: | A simple multi-residue analysis method for the quantitative determination of eprinomectin, abamectin, doramectin and ivermectin
in bovine tissues was developed. The tissue sample was extracted with acetonitrile, followed by clean-up on a C18 solid phase extraction cartridge. The eluate was derivatised before being analyzed by HPLC coupled to a fluorescence detector.
The method was validated using bovine liver and muscle fortified with the drugs at 0, 5, 10 and 50 ng g−1. The mean recoveries of the four drugs were 70.31–87.11% in liver and 79.57–93.65% in muscle, with relative standard deviations
below 17.84% in liver and 14.68% in muscle. The limits of detection were between 0.5 and 1.0 ng g−1 and the limits of quantification were 1–2 ng g−1 in bovine tissues for the four drugs. |
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| Keywords: | Column liquid chromatography Fluorescence detection Avermectins in bovine tissue Eprinomectin abamectin doramectin and Ivermectin |
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