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Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis
Authors:Ryan Chris P  Smith Mark E B  Schumacher Felix F  Grohmann Dina  Papaioannou Danai  Waksman Gabriel  Werner Finn  Baker James R  Caddick Stephen
Institution:Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK.
Abstract:Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described.
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