Mechanism of Incompatible Herb Pairs,Panax ginseng and Veratrum nigrum L.: Material Basis and Metabolic Profiles of Ginsenosides in Rat Intestinal Bacteria |
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| Authors: | Xiudong Yang Xue Li Ying Xu Zifeng Pi Na Lin Zhiqiang Liu Fengrui Song |
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| Affiliation: | 1. National Center of Mass Spectrometry in Changchun & Jilin Province, Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China;2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | ![]()
In traditional Chinese medicine theory, Panax ginseng and Veratrum nigrum L. is an important incompatible herb pair. Studies on the content variation of main components and the influences on the metabolism in rat intestinal bacteria are useful to understand the mechanism of incompatibility of this herb pairs. In this study, the content variation of ginsenosides and their metaboltic profiles in the extracts of P. ginseng and compatibility of P. ginseng with V. nigrum L. (G‐V) were investigated using relative quantitative method of electrospray ionization mass spectrometry (ESI‐MS) and UPLC‐MSn, respectively. The relative contents of most ginsenosides were reduced in the extract of G‐V. Furthermore, ginsenosides Rb1, Rb2, Rc and Rd could be metabolized to Rd, F2 and C‐K in rat intestinal bacteria. The metabolic speeds of Rb1, Rb2 and Rc in the G‐V extracts at ratios of 10:5, 10:7 and 10:10 and the metabolic rates of ginsenosides Rb1, Rb2 and Rc to Rd, Rd to F2 in all compatibility extracts were lower than that in the P. ginseng extract. In conclusion, this study illustrated the mechanism of effect‐reducing by comparison of the relative contents and metabolic profiles of ginsenosides after compatibility of P. ginseng and V. nigrum L. |
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| Keywords: | Panax ginseng Veratrum nigrum L. rat intestinal bacteria incompatibility UPLC‐MS |
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