| 含蚕豆核酮糖1,5-二磷酸羧酶化/加氧酶大亚基基因的重组质粒物理图谱的构建 |
| |
| 引用本文： | 李加,陈擎苍,张笑敏,费承伟,翁醒华,唐愫.含蚕豆核酮糖1,5-二磷酸羧酶化/加氧酶大亚基基因的重组质粒物理图谱的构建[J].浙江大学学报(理学版),1990(3). |
| |
| 作者姓名： | 李加 陈擎苍 张笑敏 费承伟 翁醒华 唐愫 |
| |
| 作者单位： | 杭州大学生物学系，杭州大学生物学系，杭州大学生物学系，杭州大学生物学系，杭州大学生物学系，杭州大学生物学系 83级学生，83级学生 |
| |
| 摘 要： | pVFB33为含蚕豆叶绿体核酮糖1,5-二磷酸羧化酶/加氧酶大亚基基因的克隆.本实验采用碱变性法,提取、分离和纯化了pVFB33质粒DNA,并利用几种限制性内切酶酶解,构建了pVFB33质粒DNA的物理图谱,同时,对实验结果进行了分析讨论.
|
| 关 键 词： | 重组DNA 物理图谱 核酮糖1 5-二磷酸羧化酶/加氧酶 蚕豆叶绿体 |
Physical Map of Recombinant Piasmid Containing the Gene for the Large Subunit of Ribulose 1,5 bisphose Phate Carboxylase/Oxygenase from Broad Bean Chloro Plast DNA |
| |
| Li Jia Chen Qinchuang Zhan Xiaoming Fei Chengwei,Weng Xinghua Tang Su.Physical Map of Recombinant Piasmid Containing the Gene for the Large Subunit of Ribulose 1,5 bisphose Phate Carboxylase/Oxygenase from Broad Bean Chloro Plast DNA[J].Journal of Zhejiang University(Sciences Edition),1990(3). |
| |
| Authors: | Li Jia Chen Qinchuang Zhan Xiaoming Fei Chengwei Weng Xinghua Tang Su |
| |
| Institution: | Department of Biology |
| |
| Abstract: | PVFB33 contains the gene for the large subunit of ribuloss-1.5-bis-phosphate carboxylase/oxygenase from broad bean cbloroplast DNA. PVFB33 plasmid DNA is isolated and purified by alkali denaturation method. A physical map of the recombinant plasmid is constucted by cleavage with several restriction endonucleases. The results are discussed. |
| |
| Keywords: | recombinant DNA physical map ribulose-3 5-bisphosphate carboxylase/oxyenasa broad bean chloroplast |
| 本文献已被 CNKI 等数据库收录！ |
