Alternate HPLC method for the analysis of tetrabutylammonium hydroxide in [18F]fluorodeoxythymidine (FLT) |
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| Authors: | Jackson Poon Andrew Katsifis Michael Fulham |
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| Institution: | 1. Department of PET and Nuclear Medicine Royal Prince Alfred Hospital, Sydney, AustraliaJackson@nucmed.rpa.cs.nsw.gov.au;3. Department of PET and Nuclear Medicine Royal Prince Alfred Hospital, Sydney, Australia |
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| Abstract: | Tetrabutylammonium hydroxide is a common reagent used in the synthesis of 18F]Fluorodeoxythymidine (FLT) for positron emission tomography imaging. The British Pharmacopeia monograph for the analysis of 18F]FLT was released in 2015 incorporating a HPLC method for the analysis of tetrabutylammonium hydroxide. We describe alternate HPLC conditions that mitigate the challenges in fulfilling the system suitability requirements such as signal to noise and symmetry factor that are specified in the monograph. Our method was validated by analyzing tetrabutylammonium hydroxide at a range of concentrations to determine the linearity (R2?=?0.994), accuracy (≤9.2%) and precision (≤3.2%). Our method does not affect the analysis of 18F]FLT and it complies with all requirements in the BP monograph. |
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| Keywords: | British Pharmacopeia HPLC [18F]Fluorodeoxythymidine [18F]FLT HPLC TBA-OH Tetrabutylammonium hydroxide |
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