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HPLC method for determination of moxifloxacin in plasma and its application in pharmacokinetic analysis |
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| Authors: | Andrzej Czyrski Agnieszka Sokó? Edyta Sza?ek |
| Institution: | 1. Department of Physical Pharmacy and Pharmacokinetics, Poznań University of Medical Sciences, Poznań, Polandaczyrski@ump.edu.pl;3. Department of Physical Pharmacy and Pharmacokinetics, Poznań University of Medical Sciences, Poznań, Poland;4. Department of Clinical Pharmacy and Biopharmacy, Poznań University of Medical Sciences, Poznań, Poland |
| Abstract: | Moxifloxacin is a representative of the fourth generation of fluoroquinolones. It possesses bacteriostatic activity against Gram-positive and Gram-negative bacteria. A simple and fast high performance liquid chromatography-ultraviolet detection (HPLC-UV) method was developed for moxifloxacin analysis. The separation was performed on C18 column. The mobile phase was a mixture of acetonitrile and 0.4% triethylamine solution. The regression curve was linear over the range 0.2–10.0?µg/mL. The validation parameters obey the European Medicine Agency limits. The in vivo study confirmed the practical application of the method. |
| Keywords: | HPLC moxifloxacin pharmacokinetics plasma therapeutic drug monitoring UV detection |