Urease-Dextran complexes with enhanced enzymatic activity and stability |
| |
| Authors: | Melda Altıkatoğlu Yapaöz Azra Destanoğlu |
| |
| Institution: | 1. Yildiz Technical University, Faculty of Arts and Sciences, Department of Chemistry, Davutpasa Campus Esenler, Istanbul, TURKEYmaltikatoglu@yahoo.com;3. Yildiz Technical University, Faculty of Arts and Sciences, Department of Chemistry, Davutpasa Campus Esenler, Istanbul, TURKEY |
| |
| Abstract: | In this study, the urease-dextran non-covalent complexes in various molar ratios were synthesized and compared to the free enzyme in terms of pH, temperature, thermal and storage stabilities. Especially, the complex with a molar ratio of nU/nDA = 40/1 showed highest thermal stability and had ca. 1.4-fold at 25°C and 2.5-fold at 80°C higher activity than the free enzyme. The complex showed a high catalytic activity in organic solvent. In addition, the thermal and storage stabilities of urease were improved greatly as dextran complex, which has advantages for usage in practice. |
| |
| Keywords: | stabilization urease dextran complex |
|
|
