| 壳聚糖/Nafion膜固定氯化血红素制备过氧化氢生物传感器 |
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| 引用本文： | 潘芊秀,王怀生,王桂香.壳聚糖/Nafion膜固定氯化血红素制备过氧化氢生物传感器[J].分析试验室,2010,29(1). |
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| 作者姓名： | 潘芊秀 王怀生 王桂香 |
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| 作者单位： | 1. 聊城大学化学化工学院分析化学研究所,聊城,252059;潍坊医学院化学教研室,潍坊,261053
2. 聊城大学化学化工学院分析化学研究所,聊城,252059 |
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| 摘 要： | 以壳聚糖/Nafion复合膜为载体在玻碳电极上固定氯化血红素制备过氧化氢生物传感器。研究了血红素电极制备过程中的影响因素及其电化学性质,在1.0 mol/L pH 9.8的NH3-NH4Cl缓冲溶液中,血红素电极对H2O2的催化还原峰电流与H2O2浓度在7.5×10-5~1.45×10-3mol/L范围内成良好的线性关系,检测灵敏度为35.28μA/mM,检测限为3.5×10-5mol/L(S/N=3),同时研究了血红素电极的重现性、稳定性和选择性。
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| 关 键 词： | 壳聚糖 Nafion 氯化血红素 过氧化氢生物传感器 |
Hydrogen peroxide biosensor based on immobilization of hemin in chitosan/nafion matrix |
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| PAN Qian-xiu,WANG Huai-sheng,WANG Gui-xiang.Hydrogen peroxide biosensor based on immobilization of hemin in chitosan/nafion matrix[J].Chinese Journal of Analysis Laboratory,2010,29(1). |
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| Authors: | PAN Qian-xiu WANG Huai-sheng WANG Gui-xiang |
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| Institution: | 1.Department of Chemistry;Institute of Analytical Chemistry;Liaocheng University;Liaocheng 252059;2.Department of Chemistry;Weifang Medical University;Weifang 261053 |
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| Abstract: | An amperometric biosensor was developed for determination of H_2O_2 based on the immobilization of hemin in chitosan/nafion composite films. The fabrication procedure and electrochemical characteristics of the hemin electrode were investigated. The catalytic current response of the hemin electrode to H_2O_2 was linear in the range of 7.5×10~(-5)～1.45×10~(-3) mol/L with a sensitivity of 35.28 μA/mM. The detection limit of the hemin electrode was found to be 3.5×10~(-5) mol/L (S/N=3). |
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| Keywords: | Chitosan Nafion Hemin Hydrogen peroxide biosensor |
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