Click Chemistry Mediated Functionalization of Vertical Nanowires for Biological Applications |
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| Authors: | Surendra Vutti Dr. Sanne Schoffelen Dr. Jessica Bolinsson Nina Buch‐Månson Prof. Nicolas Bovet Prof. Jesper Nygård Prof. Karen L. Martinez Prof. Morten Meldal |
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| Affiliation: | 1. Center for Evolutionary Chemical Biology, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark);2. Nano‐science Center and Center for Quantum Devices, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark);3. Bionanotechnology and Nanobiomedicine Laboratory, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark);4. Nano‐Science Center, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark) |
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| Abstract: | ![]()
Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI‐catalyzed alkyne–azide cycloaddition and its strain‐promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site‐specific manner and recognized by antibody binding to demonstrate the proof‐of‐concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material. |
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| Keywords: | alkyne– azide cycloaddition biosensors diazotransfer nanowires protein immobilization |
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