Determination of aristolochic acid by capillary zone electrophoresis |
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Authors: | Franti?ek Kvasni?ka Rudolf ?ev?ík Michal Vold?ich Jana Krátká |
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Institution: | (1) Institute of Chemical Technology, Technicka 1905/5, 166 28 Prague 6, Czech Republic |
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Abstract: | A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary
supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5
minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose
in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed
method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running
cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC. |
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Keywords: | aristolochic acid food supplement herbs slimming capillary zone electrophoresis |
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