Ethanolic Extract from Pteris wallichiana Alleviates DSS-Induced Intestinal Inflammation and Intestinal Barrier Dysfunction by Inhibiting the TLR4/NF-κB Pathway and Regulating Tight Junction Proteins |
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| Authors: | Junhong Tao Zhihua Huang Yudan Wang Yaping Liu Tianrui Zhao Yifen Wang Lei Tian Guiguang Cheng |
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| Affiliation: | 1.Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China; (J.T.); (Y.W.); (Y.L.); (T.Z.); (L.T.);2.Yunnan Tobacco Company, Yuxi Branch, Yuxi 653100, China;3.National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming 650500, China;4.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650000, China; |
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| Abstract: | ![]()
The aim of the research was to determine the protective effect and mechanism of Pteris wallichiana J. Agardh extract (PWE) on DSS-induced ulcerative colitis (UC) in mice. In this research, PWE is rich in flavonoids and diterpenoids by UPLC-MS/MS analysis. In LPS-induced RAW264.7 cells, PWE reduced the productions of inflammatory factors (i.e., NO, TNF-α, IL-6, and IL-1β). In DSS-induced UC in mice, PWE improved disease activity index (DAI) score, attenuated oxidative stress by decreasing MPO and MDA activities and activating GSH and SOD levels, and inhibited TNF-α, IL-6, and IL-1β expressions in the colonic tissues. PWE also improved the intestinal barrier by upregulating the expressions of tight junction proteins, including occludin and ZO-1. Moreover, PWE extract alleviated intestinal inflammation by suppressing the TLR4/MyD88/NF-κB signaling pathway. Conclusion: PWE can alleviate DSS-induced UC in mice by increasing the expressions of intestinal tight junction proteins and inhibiting the TLR4/NF-κB inflammatory pathway. |
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| Keywords: | Pteris wallichiana chemical constituents ulcerative colitis anti-inflammatory activity tight junction proteins |
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