Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate** |
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| Authors: | Ander Chapartegui-Arias Anna Raysyan Dr Ana M Belenguer Dr Carsten Jaeger Teodor Tchipilov Carsten Prinz Dr Carlos Abad Dr Sebastian Beyer Dr Rudolf J Schneider Dr Franziska Emmerling |
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| Institution: | 1. BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str.11, 12489 Berlin, Germany;2. Yusuf Hamied Department of Chemistry, University of Cambridge Lensfield Road, Cambridge, CB2 1EW United Kingdom;3. Department of Biomedical Engineering Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong Shatin, Hong Kong |
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| Abstract: | Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC-MS/MS provided evidence that a covalent link was established between these metal-organic frameworks and lysine residues in IgG antibodies. |
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| Keywords: | antibodies immunoassays metal-organic frameworks nanoparticles zeolite analogues |
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