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STED Imaging the Dynamics of Lysosomes by Dually Fluorogenic Si-Rhodamine |
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| Authors: | Mengting Fan Haiyan An Chuanfeng Wang Dr Shuhui Huo Dr Ting Wang Dr Xiaoyan Cui Dr Dazhi Zhang |
| Institution: | 1. Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241 P. R. China
Both authors contributed equally to this work.;2. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070 P. R. China Department of Organic Chemistry, College of Pharmacy, Second Military Medical University, Shanghai, 200433 P. R. China Both authors contributed equally to this work.;3. Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241 P. R. China;4. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070 P. R. China;5. Department of Organic Chemistry, College of Pharmacy, Second Military Medical University, Shanghai, 200433 P. R. China |
| Abstract: | Super-resolution microscopy (SRM) imaging of the finite subcellular structures and subtle bioactivities inside organelles delivers abundant cellular information with high fidelity to unravel the intricate biological processes. An ideal fluorescent probe with precise control of fluorescence is critical in SRM technique like stimulated emission depletion (STED). Si-rhodamine was decorated with both targeting group and H+-receptor, affording the dually fluorogenic Si-rhodamine in which the NIR fluorescence was efficiently controlled by the coalescent of spirolactone-zwitterion equilibrium and PeT mechanism. The dually fluorogenic characters of the probe offer a perfect mutual enhancement in sensitivity, specificity and spatial resolution. Strong fluorescence only released in the existence of targeting protein at acidic lysosomal pH, ensured precisely tracking the dynamic of lysosomal structure and pH in living cells by STED. |
| Keywords: | Fluorogenic lysosome microscopy Si-rhodamine STED |