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Investigation of transient two-photon excited fluorescence in biological tissue
Authors:Yishen Qiu  Enguo Chen  Gaoming Li  and Hui Li
Institution:1Key Laboratory of OptoElectronic Science and Technology for Medicine Ministry of Education,Fujian Provincial Key Laboratory for Photonics Technology,Fujian Normal University,Fuzhou 350007,China 2 Department of Optical Engineering,Zhejiang University,Hangzhou 310058,China
Abstract:The fluorescence power from biological tissue excited by a femtosecond laser pulse compared with excitation power does not appear to obey a simple quadratic relationship given by the steady non-linear theory.A more reliable analysis is developed based on transient two-photon absorption because the response time of two-photon absorption is longer than the width of a femtosecond pulse.Good agreement is obtained between the theoretical analysis and the experimental results of fluorescence power versus excitation power.This letter offers potential value to non-linear optics in biological tissues.
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