Large-Scale Isolation and Purification of Scoparone from Herba artemisiae scopariae by High-Speed Counter-Current Chromatography |
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| Authors: | C. H. Ma W. Ke Z. L. Sun J. Y. Peng Z. X. Li X. Zhou G. R. Fan C. G. Huang |
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| Affiliation: | (1) Shanghai Institute of Materia Medica, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai, 201203, China;(2) Center of Research and Development on Life Sciences and Enviromental Sciences, Harbin University of Commerce, Harbin, 150076, China;(3) Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai, 200433, China |
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| Abstract: | A preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol- water (1:1:0.45:1.55, v/v/v/v) was successfully performed to isolate scoparone (6,7-dimethoxycoumarin, 6,7-dimethylesculetin, 6,7-DME) from the plant of Herba artemisiae scopariae, a traditional Chinese medicine. 233.5 mg Scoparone with the purity of 96.8% (determined by HPLC) was obtained in one-step elution from 800 mg crude extract. The recovery of scoparone was 91.8%, and the chemical structure of this compound was identified by IR, MS, 1H NMR and 13C NMR spectrum. |
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| Keywords: | Column liquid chromatography Counter-current chromatography Coumarin compounds Scoparone Herba artemisiae scopariae Artemisia capillaries and Artemisia scoparia |
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