Effect of pH on the Metabolism of Aconitine under Rat Intestinal Bacteria and Analysis of Metabolites Using HPLC/MS-MSn Technique |
| |
| Authors: | Xiye Wang Zifeng Pi Wenlong Liu Yufeng Zhao Prof. Dr. Shuying Liu |
| |
| Affiliation: | 1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;3. Shanghai Center of System Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China |
| |
| Abstract: | A semi‐quantitative method of mass spectrometry (MS) has been described for the analysis of metabolites of aconitine by rat intestinal bacteria at different pH. At pH 7.0, the rat intestinal bacteria exhibit optimal activity for the metabolism of aconitine. A high‐performance liquid chromatography‐electrospray ionization multiple‐stage mass spectrometry (HPLC/ESI‐MSn) method has been applied to investigate the characteristic product ions of metabolites. Then, the logical fragmentation pathways of metabolites have been proposed. By comparing the retention time (tR) of HPLC and the ESI‐MSn data with the data of standard compounds and reports from literature, ten metabolites have been identified and a distinctive metabolite (15‐deoxyaconitine) has been deduced first time. The experimental results demonstrate that HPLC/ESI‐MSn is a specific and useful method for the identification of metabolites of aconitine. Also, in the present paper, the HPLC‐MS method was introduced to determine the synthetical metabolite prior to the study of the toxicity by the method of Bliss. |
| |
| Keywords: | aconitine metabolism intestinal bacteria mass spectrometry 8‐butyryl‐benzoylmesaconine |
| 本文献已被 万方数据 等数据库收录！ |
|
