Electrochemical sensing platform based on the use of ZnONPs and MWCNTs as CPE modifiers for a selective and sensitive determination of polyamine spermine in the urine sample |
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| Authors: | Zorica S Stojanović Ana D Đurović Snežana Ž Kravić Amir M Ashrafi Lukáš Richtera |
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| Institution: | 1. University of Novi Sad, Faculty of Technology Novi Sad, Department of Applied and Engineering Chemistry, Bul. cara Lazara 1, 21 000 Novi Sad, Serbia;2. Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 612 00 Brno, Czech Republic |
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| Abstract: | A simple electroanalytical procedure has been developed for the determination of polyamine spermine using the ZnONPs-MWCNTs-CPE sensor. Spermine has been irreversibly oxidized on a modified electrode at a potential of +0.92 V versus Ag/AgCl (KCl, 3.5 mol L−1). The developed sensor demonstrated a respective linear response in borate buffer pH 8.5 from 2 μmol L−1 to 100 μmol L−1, with a detection limit of 0.300 μmol L−1 and quantification limit of 0.998 μmol L−1. The proposed sensor showed high reproducibility (RSD = 2.58 %), stability, robustness, and no obvious interference effects of several inorganic ions and organic molecules. The obtained results demonstrated excellent performance during the determination of the spermine in human urine samples with satisfactory recovery results (98.41–101.34 %), offering promising opportunities for practical clinical analysis. |
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| Keywords: | modified CPE spermine urine sample voltammetry |
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