Isolation and Structural Characterization of a Second Polysaccharide from Bulbs of Lanzhou Lily |
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Authors: | Fengxia Wang Wei Wang Xiaobo Niu Yulong Huang Ji Zhang |
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Institution: | 1.College of Life Science,Northwest Normal University,Lanzhou,China;2.Bioactive Products Engineering Research Center for Gansu Distinctive Plants,Lanzhou,China;3.School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou,China |
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Abstract: | In this study, a second water-soluble polysaccharide (designated as LDP-2) was isolated from the bulbs of Lanzhou Lily (Lilium davidii var. unicolor). Based on monosaccharide composition and methylation analysis, its structural features were investigated using a variety of characterizations, such as size-exclusion chromatography combined with laser light scattering (SEC-LLS), gas chromatography-mass spectroscopy (GC-MS), infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The results demonstrate that LDP-2 is a homogeneous heteropolysaccharide (molecular weight 6.2?×?104 Da), which includes four kinds of monosaccharides (Lyx, Man, Glc, and Gal in an approximate weight ratio of 6.74: 6.28: 76.50: 10.48). Furthermore, its morphology, thermal behavior, and preliminary hypoglycemic activities were also investigated. |
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