Production of Fumaric Acid by Bioconversion of Corncob Hydrolytes Using an Improved <Emphasis Type="Italic">Rhizopus oryzae</Emphasis> Strain |
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| Authors: | Xuefeng Wu Qing Liu Yongdong Deng Xiaoju Chen Zhi Zheng Shaotong Jiang Xingjiang Li |
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| Institution: | 1.School of Food Science and Engineering,Hefei University of Technology,Hefei,China;2.Key Laboratory for Agricultural Products Processing of Anhui Province,Hefei,China;3.College of Chemistry and Material Engineering,Chaohu University,Hefei,China |
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| Abstract: | The use of microorganism fermentation for production of fumaric acid (FA), which is widely used in food, medicine, and other fields, can provide technical support for the FA industry. In this study, we aimed to increase the titer of FA production by using an improved Rhizopus oryzae WHT5, which was domesticated to obtain a furfural-resistant strain in corncob hydrolytes. The metabolic pathways and metabolic network of this strain were investigated, and the related enzymes and metabolic flux were analyzed. Metabolic pathway analysis showed that the R. oryzae WHT5 strain produced FA mainly through two pathways. One occurred in the cytoplasm and the other was a mitochondrial pathway. The key parameters of the fermentation process were analyzed. The FA titer was 49.05 g/L from corncob hydrolytes using R. oryzae WHT5 in a 7-L bioreactor. The use of a furfural-resistant strain developed through domestication effectively increased the titer of FA. This capacity of the microorganisms to produce high amounts of FA by bioconverting corncob hydrolyte can be further applied for industrial production of FA. |
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