白藜芦醇探针同步荧光法测定蛋白质含量

在Tris-HCl缓冲溶液体系中(pH=7.4)，白藜芦醇与人血清白蛋白相互作用，对蛋白的内源性荧光产生猝灭作用，而且，同步荧光的强度与人血清白蛋白的浓度成正比。 基于此，建立了以白藜芦醇为荧光探针，运用固定波长同步荧光光谱法测定生物样品中蛋白质含量的新方法。 体系同步荧光光谱特征及强度受Δλ、反应介质和离子强度等因素的影响。 在最佳实验条件下，体系的同步荧光强度(I)与人血清白蛋白在1.380~276.0 mg/L的浓度范围内呈良好的线性关系，检测限为1.037 mg/L(n=11)。 对血清、尿样和唾液等生物样品进行测定，回收率在93.5%~105.8%之间，与传统的考马斯亮蓝(G-250)法作对照，结果一致。

Synchronous Fluorescence Spectrometric Determination of Proteins with Resveratrol as Probe

The three-dimensional spectra combined with fluorescence spectroscopy under simulative physiological conditions were used to investigate the interaction between resveratrol and human serum albumin.With the presence of Tris-HCl,the endogenous fluorescence was quenched by resveratrol and the three-dimensional spectra also changed when resveratrol exsited in the system.The synchronous fluorescence intensity and the concentration of serum albumin solution have a good linear relationship.A new synchronous fluore...