A modular microfluidic system for deoxyribonucleic acid identification by short tandem repeat analysis |
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Authors: | Reedy Carmen R Hagan Kristin A Marchiarullo Daniel J Dewald Alison H Barron Annalise Bienvenue Joan M Landers James P |
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Institution: | aDepartment of Chemistry, University of Virginia, Charlottesville, VA 22904, United States;bDepartment of Bioengineering, Stanford University, Stanford, CA 94305, United States;cLockheed Martin Corporation, Rockville, MD, United States;dDepartment of Mechanical Engineering, University of Virginia, Charlottesville, VA 22904, United States;eDepartment of Pathology, University of Virginia Health Science Center, Charlottesville, VA 22908, United States |
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Abstract: | Microfluidic technology has been utilized in the development of a modular system for DNA identification through STR (short tandem repeat) analysis, reducing the total analysis time from the ∼6 h required with conventional approaches to less than 3 h. Results demonstrate the utilization of microfluidic devices for the purification, amplification, separation and detection of 9 loci associated with a commercially-available miniSTR amplification kit commonly used in the forensic community. First, DNA from buccal swabs purified in a microdevice was proven amplifiable for the 9 miniSTR loci via infrared (IR)-mediated PCR (polymerase chain reaction) on a microdevice. Microchip electrophoresis (ME) was then demonstrated as an effective method for the separation and detection of the chip-purified and chip-amplified DNA with results equivalent to those obtained using conventional separation methods on an ABI 310 Genetic Analyzer. The 3-chip system presented here demonstrates development of a modular, microfluidic system for STR analysis, allowing for user-discretion as to how to proceed after each process during the analysis of forensic casework samples. |
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Keywords: | Microfluidics Short tandem repeat analysis Deoxyribonucleic acid identification Forensics |
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