Ultra-sensitive DNA assay based on single-molecule detection coupled with fluorescent quantum dot-labeling and its application to determination of messenger RNA |
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| Authors: | Li Li Xincang Li Lu Li Jinxing Wang Wenrui Jin |
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| Affiliation: | aSchool of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;bSchool of Life Sciences, Shandong University, Jinan 250100, China |
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| Abstract: | ![]()
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| Keywords: | Abbreviations: BSA, bovine serum albumin DEPC, diethypyrocarbonate DNase, deoxyribonuclease dNTP, deoxy-ribonucleoside triphosphate DSC, decidual stromal cell GOPS, 3-glycidoxypropyltrimethoxysilane mRNA, messenger RNA OPN, osteopontin PBS, physiological buffer saline pDNA, probe DNA QD, quantum dot QD-pDNA, QD-labeled probe DNA R6G, rhodamine 6G RNase, ribonuclease rRNAsin, RNase-inhibitor SA-QD, Streptavidin-coated QD SDS, sodium dodecyl sulfate SMD, single-molecule detection tDNA, target DNA TIRFM, total internal reflection fluorescence microscopy |
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