Radiation sterilization of enzyme hybrids with biodegradable polymers |
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Authors: | Masakazu Furuta Masahito Oka Toshio Hayashi |
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Institution: | Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1-2, Gakuen-cho, Sakai, Osaka 599-8570, Japan |
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Abstract: | Ionizing radiations, which have already been utilized for the sterilization of medical supplies as well as gas fumigation, should be the final candidate to decontaminate “hybrid” biomaterials containing bio-active materials including enzymes because irradiation induces neither heat nor substances affecting the quality of the materials and our health. In order to check the feasibility of 60Co-gamma rays on these materials, we selected commercial proteases including papain and bromelain hybridized with commercial activated chitosan beads and demonstrated that these enzyme-hybrids suspended in water showed the significant radiation durability of more than twice as much as free enzyme solution at 25-kGy irradiation. Enhanced thermal and storage stability of the enzyme hybrids were not affected by the same dose level of irradiation, either, indicating that commercial irradiation sterilization method is applicable to enzyme hybrids without modification. |
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Keywords: | Hybrid 60Co-gamma rays Papain Bromelain Storage stability Sterilization |
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