QUENCHING OF PYRENE FLUORESCENCE BY TRYPTOPHAN IN MICELLAR SOLUTIONS |
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| Authors: | M. V. Encinas E. A. Lissi |
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| Affiliation: | Departamento de Química, Facultad de Ciencias, Universidad de Santiago de Chile, Santiago, Chile |
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| Abstract: | Deactivation of excited pyrene incorporated to cationic (cetyltrimethylammonium chloride), anionic (sodium dodecyl sulfate) and neutral (Triton X-100) micelles by tryptophan has been investigated over a wide pH range. Data obtained allow an estimation of the tryptophan association to the micelles, of the tryptophan apparent p K in the micellar solutions, and of the dynamics of tryptophan incorporation to the micellar pseudophase. In particular, the data obtained at pH 7 allow an estimation of the effect of the micellar charge upon the binding capacity of the tryptophan zwitterion. |
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