High-speed synthetic aperture microscopy for live cell imaging |
| |
Authors: | Kim Moonseok Choi Youngwoon Fang-Yen Christopher Sung Yongjin Dasari Ramachandra R Feld Michael S Choi Wonshik |
| |
Institution: | Department of Physics, Korea University, Seoul 136-701, South Korea. |
| |
Abstract: | We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|