Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping |
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| Authors: | Saito Yoshio Bag Subhendu Sekhar Kusakabe Yuichi Nagai Chiharu Matsumoto Katsuhiko Mizuno Erika Kodate Satoshi Suzuka Isamu Saito Isao |
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| Affiliation: | Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saitoy@chem.ce.nihon-u.ac.jp |
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| Abstract: | ![]()
A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, (Py)U/(2-Ant)U, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at the donor, only when the opposite base of the "smart" fluorescently labeled BDF nucleoside, (Py)U/(2-Ant)U, is adenine on the complementary target sequence. |
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