Determination of glucosinolates in traditional Chinese herbs by high-performance liquid chromatography and electrospray ionization mass spectrometry |
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Authors: | Kim-Chung Lee Man-Wai Cheuk Wan Chan Albert Wai-Ming Lee Zhong-Zhen Zhao Zhi-Hong Jiang Zongwei Cai |
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Institution: | (1) Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, People’s Republic of China;(2) School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, People’s Republic of China |
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Abstract: | A reversed-phase HPLC method has been developed for determination of twelve intact glucosinolates—glucoiberin, glucocheirolin,
progoitrin, sinigrin, epiprogoitrin, glucoraphenin, sinalbin, gluconapin, glucosibarin, glucotropaeolin, glucoerucin, and
gluconasturtiin—in ten traditional Chinese plants. The samples were extracted with methanol and the extracts were cleaned
on an activated Florisil column. A mobile phase gradient prepared from methanol and 30 mmol L−1 ammonium acetate at pH 5.0 enabled baseline separation of the glucosinolates. Glucosinolate detection was confirmed by quadrupole
time-of-flight tandem mass spectrometric analysis in negative-ionization mode. Detection limits ranged from 0.06 to 0.36 μg
g−1 when 5 g of dried plant was analyzed. Recoveries of the glucosinolates were better than 85% and precision (relative standard
derivation, n = 3) ranged from 5.3 to 14.6%. Analysis of the glucosinolates provided scientific evidence enabling differentiation of three
pairs of easily confused plants.
Figure Glucosinolates Analysis for the Differentiation of Easily-Confusing Herbs |
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Keywords: | Glucosinolate Traditional Chinese herb HPLC ESI– MS |
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